Two dimensional mass spectroscopy

May 15, 2007

Title: Single-molecule mass spectroscopy in solution using a solitary nanopore

Authors: Joseph W F Robertson, Claudio G Rodrigues, Vincent M Stanford, Kenneth A Rubinson, Oleg V Krasilnikov, and John J Kasianowicz

Source: PNAS, May 15, 2007, Vol. 104, No. 20, pp. 8207-8211

Abstract: We introduce a two-dimensional method for mass spectrometry in solution that is based on the interaction between a nanometer-scale pore and analytes. As an example, poly(ethylene glycol) molecules that enter a single {alpha}-hemolysin pore cause distinct mass-dependent conductance states with characteristic mean residence times. The conductance-based mass spectrum clearly resolves the repeat unit of ethylene glycol, and the mean residence time increases monotonically with the poly(ethylene glycol) mass. This technique could prove useful for the real-time characterization of molecules in solution.

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