Two dimensional mass spectroscopy
May 15, 2007
Title: Single-molecule mass spectroscopy in solution using a solitary nanopore
Authors: Joseph W F Robertson, Claudio G Rodrigues, Vincent M Stanford, Kenneth A Rubinson, Oleg V Krasilnikov, and John J Kasianowicz
Source: PNAS, May 15, 2007, Vol. 104, No. 20, pp. 8207-8211
Abstract: We introduce a two-dimensional method for mass spectrometry in solution that is based on the interaction between a nanometer-scale pore and analytes. As an example, poly(ethylene glycol) molecules that enter a single {alpha}-hemolysin pore cause distinct mass-dependent conductance states with characteristic mean residence times. The conductance-based mass spectrum clearly resolves the repeat unit of ethylene glycol, and the mean residence time increases monotonically with the poly(ethylene glycol) mass. This technique could prove useful for the real-time characterization of molecules in solution.
